The Human P-gp (Permeability Glycoprotein) CLIA Kit is a reliable and accurate tool for the detection of P-gp levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.P-gp is a vital protein that plays a crucial role in drug transport and resistance mechanisms, making it an important biomarker in pharmacology and drug development studies.
Understanding P-gp expression levels can help researchers predict drug response and optimize treatment strategies for various diseases.By using the Human P-gp CLIA Kit, researchers can effectively study P-gp function, evaluate drug interactions, and explore potential therapeutic targets related to drug resistance and transport mechanisms. This kit provides a valuable tool for advancing research in drug discovery, personalized medicine, and clinical diagnostics.
Product Name:
Human P-GP (Permeability Glycoprotein) CLIA Kit
SKU:
HUES00546
Target:
Human P-GP (Permeability Glycoprotein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human P-GP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human P-GP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human P-GP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human P-GP. You can calculate the concentration of Human P-GP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-102
98-115
88-103
Average (%)
96
105
96
1:4
Range (%)
95-109
93-105
85-99
Average (%)
101
98
92
1:8
Range (%)
96-111
93-109
96-111
Average (%)
102
100
102
1:16
Range (%)
98-111
95-107
98-115
Average (%)
104
102
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-101
96
EDTA plasma (n=5)
96-108
102
Cell culture media (n=5)
95-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
188.87
629.59
1932.34
200.43
570.49
1886.72
Standard deviation
20.68
68.56
122.7
19.32
43.13
204.9
C V (%)
10.95
10.89
6.35
9.64
7.56
10.86
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human P-GP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human P-GP in samples. No significant cross-reactivity or interference between Human P-GP and analogues was observed.
