Human PA2G4 (Proliferation Associated Protein 2G4) CLIA Kit
The Human PA2G4 (Proliferation-Associated Protein 2G4) CLIA Kit is a powerful tool for the precise measurement of PA2G4 levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it perfect for a variety of research purposes.PA2G4 is a key protein that plays a crucial role in cell proliferation and is associated with various diseases such as cancer, diabetes, and neurodegenerative disorders.
By studying PA2G4 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.This CLIA kit provides a reliable and efficient way to assess PA2G4 levels, offering researchers a valuable tool for advancing their research in the field of cell proliferation and disease biology.
Product Name:
Human PA2G4 (Proliferation Associated Protein 2G4) CLIA Kit
SKU:
HUES00523
Target:
Human PA2G4 (Proliferation Associated Protein 2G4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PA2G4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PA2G4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PA2G4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PA2G4. You can calculate the concentration of Human PA2G4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-109
91-106
85-97
Average (%)
102
97
91
1:4
Range (%)
102-118
99-114
100-112
Average (%)
110
105
106
1:8
Range (%)
95-107
89-102
99-114
Average (%)
101
95
105
1:16
Range (%)
100-112
97-112
99-113
Average (%)
105
104
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-110
102
EDTA plasma (n=5)
87-102
94
Cell culture media (n=5)
88-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
207.74
374.85
1516.52
190.92
361.0
1608.16
Standard deviation
22.31
41.31
128.9
19.74
34.55
172.07
C V (%)
10.74
11.02
8.5
10.34
9.57
10.7
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PA2G4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PA2G4 in samples. No significant cross-reactivity or interference between Human PA2G4 and analogues was observed.
