The Human PAP (Plasmin-Antiplasmin Complex) CLIA Kit is specifically designed for the accurate quantification of PAP levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.The Plasmin-Antiplasmin Complex is a critical component in the regulation of fibrinolysis, playing a key role in the breakdown of blood clots. Dysregulation of this complex has been associated with various cardiovascular and thrombotic disorders, making it a valuable biomarker for understanding these conditions and potential therapeutic interventions.
Overall, the Human PAP (Plasmin-Antiplasmin Complex) CLIA Kit provides researchers with a powerful tool for studying fibrinolysis and its role in health and disease, offering accurate and reproducible results for advanced research applications.
Product Name:
Human PAP (Plasmin-Antiplasmin Complex) CLIA Kit
SKU:
HUES01120
Target:
Human PAP (Plasmin-Antiplasmin Complex)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PAP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PAP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PAP. You can calculate the concentration of Human PAP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
98-113
85-101
Average (%)
92
106
92
1:4
Range (%)
91-104
89-102
87-100
Average (%)
96
96
94
1:8
Range (%)
97-111
88-102
92-106
Average (%)
103
95
99
1:16
Range (%)
102-115
103-117
87-98
Average (%)
108
110
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
99
EDTA plasma (n=5)
92-108
99
Cell culture media (n=5)
90-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.95
4.76
13.91
2.08
4.82
12.89
Standard deviation
0.22
0.43
0.86
0.19
0.38
0.91
C V (%)
11.28
9.03
6.18
9.13
7.88
7.06
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PAP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PAP in samples. No significant cross-reactivity or interference between Human PAP and analogues was observed.
