Human PAPPA (Pregnancy Associated Plasma Protein A) CLIA Kit
The Human PAPPA (Pregnancy-associated plasma protein A) CLIA Kit is a comprehensive solution for the accurate measurement of PAPPA levels in human samples. This kit offers high sensitivity and specificity, guaranteeing precise and consistent results for various research endeavors.PAPPA is a critical protein that plays a vital role in pregnancy and fetal development. It is involved in the regulation of insulin-like growth factor bioavailability, making it a valuable biomarker for monitoring pregnancy-related complications and potentially diagnosing fetal abnormalities.
With the Human PAPPA CLIA Kit, researchers can easily quantify PAPPA levels in serum, plasma, and other biological samples, enabling a deeper understanding of its physiological functions and its potential implications in various medical conditions.
Product Name:
Human PAPPA (Pregnancy Associated Plasma Protein A) CLIA Kit
SKU:
HUES00129
Target:
Human PAPPA (Pregnancy Associated Plasma Protein A)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PAPPA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PAPPA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PAPPA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PAPPA. You can calculate the concentration of Human PAPPA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
89-102
99-111
Average (%)
92
95
104
1:4
Range (%)
102-119
91-103
90-104
Average (%)
109
96
96
1:8
Range (%)
94-110
91-103
84-97
Average (%)
101
98
91
1:16
Range (%)
101-115
100-115
98-111
Average (%)
107
107
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-114
105
EDTA plasma (n=5)
91-105
99
Cell culture media (n=5)
85-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.46
4.91
0.51
1.37
4.54
Standard deviation
0.05
0.17
0.36
0.05
0.11
0.51
C V (%)
10.0
11.64
7.33
9.8
8.03
11.23
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PAPPA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PAPPA in samples. No significant cross-reactivity or interference between Human PAPPA and analogues was observed.
