The Human Paxillin (PAX) ELISA Kit is expertly designed for the precise measurement of Paxillin levels in human biological samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures the delivery of accurate and consistent results, perfect for a diverse range of research applications.Paxillin is a key signaling molecule that plays a critical role in cell adhesion, migration, and cytoskeletal organization. Aberrant levels of Paxillin have been linked to various diseases, including cancer, cardiovascular disorders, and neurological conditions.
As such, Paxillin serves as a valuable biomarker for the study of these diseases and the development of potential therapeutic interventions.For reliable and comprehensive analysis of Paxillin expression, the Human Paxillin (PAX) ELISA Kit is the ideal choice. Trust in its superior performance to advance your research efforts and uncover new insights into the role of Paxillin in health and disease. Visit www.assaygenie.com/human-paxillin-pax-elisa-kit/ to learn more and order your kit today.
Product Name:
Human Paxillin (Pax) ELISA Kit
Product Code:
HUDL02198
Size:
96 Assays
Target:
Pax
Synonyms:
PXN
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
