Human PCDH beta16 (Protocadherin Beta 16) CLIA Kit
The Human PCDH-Beta16 (Protocadherin Beta-16) CLIA Kit is specifically designed for the accurate measurement of PCDH-Beta16 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research purposes.PCDH-Beta16 is a vital protein that is involved in cell-to-cell adhesion and communication, playing a crucial role in the development and function of the nervous system.
Dysregulation of PCDH-Beta16 has been linked to various neurological disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.With its advanced technology and robust performance, the Human PCDH-Beta16 CLIA Kit is an essential tool for researchers investigating the role of Protocadherin Beta-16 in health and disease.
Product Name:
Human PCDH beta16 (Protocadherin Beta 16) CLIA Kit
SKU:
HUES00558
Target:
Human PCDH beta16 (Protocadherin Beta 16)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PCDHβ16. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PCDHβ16 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PCDHβ16, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PCDHβ16. You can calculate the concentration of Human PCDHβ16 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
96-109
100-116
Average (%)
98
101
109
1:4
Range (%)
100-116
101-115
96-113
Average (%)
108
109
103
1:8
Range (%)
92-107
86-97
102-116
Average (%)
99
91
108
1:16
Range (%)
92-108
100-114
94-108
Average (%)
98
106
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-107
98
EDTA plasma (n=5)
95-109
102
Cell culture media (n=5)
96-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.27
303.65
718.47
96.97
291.61
709.93
Standard deviation
10.36
35.71
51.87
11.09
22.25
63.54
C V (%)
10.03
11.76
7.22
11.44
7.63
8.95
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PCDHβ16 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PCDHβ16 in samples. No significant cross-reactivity or interference between Human PCDHβ16 and analogues was observed.
