Human PCNA (Proliferating Cell Nuclear Antigen) CLIA Kit
The Human PCNA (Proliferating Cell Nuclear Antigen) CLIA Kit is a highly reliable and accurate assay designed for the detection of PCNA levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers consistent and reproducible results, making it an excellent tool for researchers in various fields.PCNA is a protein essential for DNA replication and repair, functioning as a marker of cell proliferation. Its expression is increased in rapidly dividing cells, making it a valuable biomarker for studying cancer, cell growth, and DNA synthesis.
By measuring PCNA levels, researchers can gain insights into cell proliferation processes and potential therapeutic targets for various diseases.Overall, the Human PCNA CLIA Kit offers a reliable and precise method for quantifying PCNA levels in human samples, providing valuable information for research applications related to cell proliferation, cancer, and other fields of study.
Product Name:
Human PCNA (Proliferating Cell Nuclear Antigen) CLIA Kit
SKU:
HUES01246
Target:
Human PCNA (Proliferating Cell Nuclear Antigen)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PCNA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PCNA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PCNA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PCNA. You can calculate the concentration of Human PCNA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
90-104
88-100
Average (%)
102
96
95
1:4
Range (%)
96-111
85-100
95-109
Average (%)
104
91
102
1:8
Range (%)
91-106
89-101
93-108
Average (%)
97
94
100
1:16
Range (%)
96-108
93-107
102-116
Average (%)
102
99
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-109
103
EDTA plasma (n=5)
90-108
98
Cell culture media (n=5)
84-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
216.97
430.99
1672.32
207.91
412.45
1759.76
Standard deviation
25.99
47.67
105.02
25.37
45.78
113.86
C V (%)
11.98
11.06
6.28
12.2
11.1
6.47
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PCNA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PCNA in samples. No significant cross-reactivity or interference between Human PCNA and analogues was observed.
