The Human PCX (Podocalyxin) CLIA Kit is a cutting-edge diagnostic tool specifically designed for the quantitative measurement of podocalyxin levels in human samples such as serum, plasma, and cell culture supernatants. This advanced kit boasts unparalleled sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Podocalyxin is a key player in cell adhesion and migration processes, essential for normal kidney function and implicated in various diseases including cancer and diabetes.
The ability to accurately measure podocalyxin levels using this CLIA kit provides researchers with valuable insights into disease mechanisms and potential therapeutic targets.Overall, the Human PCX (Podocalyxin) CLIA Kit is a versatile and reliable tool for studying podocalyxin biology and its implications in health and disease. Its high-performance features make it an indispensable asset for researchers looking to deepen their understanding of podocalyxin's role in physiological processes and pathological conditions.
Product Name:
Human PCX (Podocalyxin) CLIA Kit
SKU:
HUES01217
Target:
Human PCX (Podocalyxin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PCX. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PCX and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PCX, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PCX. You can calculate the concentration of Human PCX in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
99-113
94-109
Average (%)
96
107
100
1:4
Range (%)
90-102
95-111
103-119
Average (%)
95
101
109
1:8
Range (%)
100-114
89-102
100-112
Average (%)
108
96
106
1:16
Range (%)
98-110
99-113
102-118
Average (%)
104
107
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
94
EDTA plasma (n=5)
89-102
94
Cell culture media (n=5)
93-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.27
300.96
813.39
111.32
312.48
741.42
Standard deviation
12.13
22.63
57.51
13.6
28.0
73.55
C V (%)
11.52
7.52
7.07
12.22
8.96
9.92
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PCX concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PCX in samples. No significant cross-reactivity or interference between Human PCX and analogues was observed.
