Human PDE4D (Phosphodiesterase 4D, cAMP Specific) CLIA Kit
The Human PDE4D (Phosphodiesterase 4D) cAMP-Specific CLIA Kit is a cutting-edge tool for precise measurement of PDE4D levels in human biological samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.PDE4D is a key enzyme involved in the regulation of cellular signaling pathways, particularly those related to cyclic AMP (cAMP) levels. Dysregulation of PDE4D has been implicated in various diseases, including inflammatory disorders, immune system dysfunction, and neurodegenerative conditions.
Therefore, the measurement of PDE4D levels is crucial for understanding disease pathogenesis and developing targeted therapies.With its advanced technology and reliable performance, the Human PDE4D cAMP-Specific CLIA Kit is a valuable asset for researchers studying PDE4D function and its implications in human health and disease. Get accurate and reproducible data with this innovative kit from Assay Genie.
Product Name:
Human PDE4D (Phosphodiesterase 4D, cAMP Specific) CLIA Kit
SKU:
HUES01271
Target:
Human PDE4D (Phosphodiesterase 4D, cAMP Specific)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PDE4D. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PDE4D and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDE4D, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PDE4D. You can calculate the concentration of Human PDE4D in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-110
92-106
96-108
Average (%)
101
98
103
1:4
Range (%)
97-111
85-99
85-99
Average (%)
104
92
91
1:8
Range (%)
99-113
95-109
102-115
Average (%)
107
101
109
1:16
Range (%)
99-112
98-111
104-116
Average (%)
106
105
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-101
93
EDTA plasma (n=5)
97-110
103
Cell culture media (n=5)
98-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
198.98
541.46
1580.65
182.0
569.31
1717.96
Standard deviation
16.06
44.13
139.73
20.49
56.36
165.61
C V (%)
8.07
8.15
8.84
11.26
9.9
9.64
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PDE4D concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Human PDE4D in samples. No significant cross-reactivity or interference between Human PDE4D and analogues was observed.
