Human PDGFD (Platelet Derived Growth Factor D) ELISA Kit
The Human PDGF-D (Platelet-Derived Growth Factor-D) ELISA Kit is a powerful tool for the accurate measurement of PDGF-D levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results that are perfect for a variety of research applications.PDGF-D is a key growth factor that plays a critical role in promoting cell growth, proliferation, and migration.
Its involvement in various biological processes makes it a valuable target for studying conditions such as cancer, wound healing, and fibrotic diseases. The Human PDGF-D ELISA Kit provides researchers with a reliable method for quantifying PDGF-D levels, facilitating in-depth investigations into its potential therapeutic applications.
Product Name:
Human PDGFD (Platelet Derived Growth Factor D) ELISA Kit
SKU:
HUES03105
Target:
Human PDGFD (Platelet Derived Growth Factor D)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PDGF-D. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PDGF-D and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDGF-D, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PDGF-D. You can calculate the concentration of Human PDGF-D in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-98
85-97
100-112
Average (%)
91
90
105
1:4
Range (%)
91-107
87-98
84-96
Average (%)
98
92
88
1:8
Range (%)
89-101
80-94
88-102
Average (%)
96
86
95
1:16
Range (%)
89-105
83-95
89-101
Average (%)
97
89
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-99
91
EDTA plasma (n=5)
85-96
91
Cell culture media (n=5)
86-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
42.48
111.87
335.2
39.82
115.27
303.8
Standard deviation
2.8
6.06
15.69
2.75
6.66
13.22
C V (%)
6.59
5.42
4.68
6.91
5.78
4.35
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PDGF-D concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PDGF-D in samples. No significant cross-reactivity or interference between Human PDGF-D and analogues was observed.
