Based on the information from www.assaygenie.com/human-pdgfr-alpha-platelet-derived-growth-factor-receptor-alpha-clia-kit-hues01164/:The Human PDGFR-Alpha (Platelet-Derived Growth Factor Receptor-Alpha) CLIA Kit is a cutting-edge assay designed for the precise measurement of PDGFR-Alpha levels in human samples. This kit offers unparalleled sensitivity and specificity, ensuring accurate and reliable results for various research applications.PDGFR-Alpha is a key receptor involved in cell signaling pathways that regulate cell growth, proliferation, and differentiation.
Its dysregulation is associated with cancer, fibrosis, and other diseases, making it a valuable target for drug development and personalized medicine approaches.With the Human PDGFR-Alpha CLIA Kit, researchers can gain valuable insights into PDGFR-Alpha expression levels and activity in different biological samples, advancing our understanding of disease mechanisms and potential therapeutic interventions.
Human PDGFR alpha (Platelet Derived Growth Factor Receptor Alpha)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PDGFRα. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PDGFRα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDGFRα, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PDGFRα. You can calculate the concentration of Human PDGFRα in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-98
102-118
95-111
Average (%)
90
108
101
1:4
Range (%)
87-101
88-101
101-114
Average (%)
93
93
106
1:8
Range (%)
92-108
88-101
98-111
Average (%)
100
95
105
1:16
Range (%)
94-107
93-105
83-95
Average (%)
99
99
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
95
EDTA plasma (n=5)
93-111
101
Cell culture media (n=5)
84-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.27
189.69
704.85
97.94
173.05
661.86
Standard deviation
10.08
21.06
62.66
11.87
19.95
58.24
C V (%)
9.58
11.1
8.89
12.12
11.53
8.8
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PDGFRα concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PDGFRα in samples. No significant cross-reactivity or interference between Human PDGFRα and analogues was observed.
