Human PDIA3 (Protein Disulfide Isomerase A3) ELISA Kit
The Human PDIA3 (Protein Disulfide Isomerase A3) ELISA Kit is specifically designed for the accurate measurement of PDIA3 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.PDIA3, also known as Protein Disulfide Isomerase A3, plays a critical role in protein folding and quality control within the endoplasmic reticulum. It is involved in various cellular processes, including protein synthesis, transport, and degradation.
Dysregulation of PDIA3 has been linked to diseases such as cancer, neurodegenerative disorders, and inflammatory conditions, making it a valuable biomarker for studying these diseases and potential therapeutic targets.Overall, the Human PDIA3 ELISA Kit from Assay Genie provides a powerful tool for researchers to investigate the role of PDIA3 in health and disease, with the potential for advancing our understanding of various pathological conditions and developing new treatment strategies.
Product Name:
Human PDIA3 (Protein Disulfide Isomerase A3) ELISA Kit
SKU:
HUES02249
Target:
Human PDIA3 (Protein Disulfide Isomerase A3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PDIA3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PDIA3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDIA3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PDIA3. You can calculate the concentration of Human PDIA3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
89-101
85-97
Average (%)
102
95
91
1:4
Range (%)
91-104
85-98
88-103
Average (%)
99
90
94
1:8
Range (%)
91-107
83-94
84-96
Average (%)
98
88
90
1:16
Range (%)
92-107
83-97
86-97
Average (%)
98
89
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-96
90
EDTA plasma (n=5)
93-107
100
Cell culture media (n=5)
87-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
0.98
4.44
0.46
0.9
4.32
Standard deviation
0.03
0.05
0.14
0.02
0.04
0.18
C V (%)
6.52
5.1
3.15
4.35
4.44
4.17
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PDIA3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PDIA3 in samples. No significant cross-reactivity or interference between Human PDIA3 and analogues was observed.
