Human PDIA6 (Protein Disulfide Isomerase A6) CLIA Kit
The Human PDIA6 (Protein Disulfide Isomerase A6) CLIA Kit is a high-quality assay kit designed for the precise quantification of PDIA6 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.PDIA6, also known as protein disulfide isomerase A6, is a key enzyme involved in the formation and regulation of disulfide bonds in proteins. It plays a crucial role in protein folding, cellular redox homeostasis, and immune responses.
Dysregulation of PDIA6 has been linked to various diseases including cancer, neurodegenerative disorders, and autoimmune diseases, making it a valuable biomarker for disease research and therapy development.The Human PDIA6 CLIA Kit from Assay Genie provides researchers with a reliable tool for studying the role of PDIA6 in health and disease. With its high accuracy and reproducibility, this kit is an essential resource for understanding the molecular mechanisms underlying various pathological conditions and exploring potential therapeutic targets.
Product Name:
Human PDIA6 (Protein Disulfide Isomerase A6) CLIA Kit
SKU:
HUES00888
Target:
Human PDIA6 (Protein Disulfide Isomerase A6)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PDIA6. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PDIA6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDIA6, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PDIA6. You can calculate the concentration of Human PDIA6 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-103
93-106
85-96
Average (%)
97
99
90
1:4
Range (%)
91-104
98-112
92-104
Average (%)
96
106
97
1:8
Range (%)
96-112
85-98
97-110
Average (%)
102
92
102
1:16
Range (%)
89-101
102-117
94-108
Average (%)
96
109
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
93
EDTA plasma (n=5)
91-106
97
Cell culture media (n=5)
95-113
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
93.48
269.54
931.7
100.71
269.13
861.43
Standard deviation
9.41
22.1
57.95
11.65
26.99
60.9
C V (%)
10.07
8.2
6.22
11.57
10.03
7.07
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PDIA6 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PDIA6 in samples. No significant cross-reactivity or interference between Human PDIA6 and analogues was observed.
