Human PDK4 (Pyruvate Dehydrogenase Kinase Isozyme 4) ELISA Kit
The Human PDK4 (Pyruvate Dehydrogenase Kinase Isozyme 4) ELISA Kit is specifically designed for the precise measurement of PDK4 levels in human biological samples such as serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers accurate and consistent results, making it a valuable tool for various research purposes.PDK4 is an important enzyme that regulates the pyruvate dehydrogenase complex, a key player in the regulation of glucose metabolism.
Dysregulation of PDK4 has been linked to metabolic disorders such as diabetes, obesity, and cancer, making it a crucial target for understanding these diseases and developing potential therapeutic interventions.Overall, the Human PDK4 ELISA Kit offers researchers a reliable and efficient method for studying the role of PDK4 in various physiological and pathological processes, contributing to advancements in the fields of metabolism, diabetes, and oncology.
Product Name:
Human PDK4 (Pyruvate Dehydrogenase Kinase Isozyme 4) ELISA Kit
SKU:
HUES02186
Target:
Human PDK4 (Pyruvate Dehydrogenase Kinase Isozyme 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PDK4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PDK4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDK4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PDK4. You can calculate the concentration of Human PDK4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
96-110
83-98
Average (%)
102
104
90
1:4
Range (%)
89-105
82-92
83-99
Average (%)
96
87
90
1:8
Range (%)
88-101
82-97
87-98
Average (%)
94
89
92
1:16
Range (%)
93-107
81-92
88-103
Average (%)
99
87
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
101
EDTA plasma (n=5)
86-98
93
Cell culture media (n=5)
87-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.76
13.42
48.89
4.95
14.36
53.28
Standard deviation
0.31
0.65
1.98
0.28
0.85
2.79
C V (%)
6.51
4.84
4.05
5.66
5.92
5.24
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PDK4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PDK4 in samples. No significant cross-reactivity or interference between Human PDK4 and analogues was observed.
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