The Human PDPN (Podoplanin) CLIA Kit is specifically designed for the precise measurement of podoplanin levels in human samples, including serum, plasma, and cell culture supernatants. This high-quality kit offers exceptional sensitivity and specificity, ensuring dependable and reproducible results for a variety of research purposes.Podoplanin is a key protein involved in various biological processes, including cell migration, adhesion, and cancer metastasis. Its expression has been linked to tumorigenesis and is considered a valuable biomarker for studying cancer progression and developing targeted therapies.
With its advanced technology and reliable performance, the Human PDPN (Podoplanin) CLIA Kit from Assay Genie is an essential tool for researchers and scientists seeking to explore the role of podoplanin in disease pathology and therapeutic interventions. Order now to advance your research efforts in oncology and beyond.
Product Name:
Human PDPN (Podoplanin) CLIA Kit
SKU:
HUES01002
Target:
Human PDPN (Podoplanin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PDPN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PDPN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDPN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PDPN. You can calculate the concentration of Human PDPN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-114
98-108
90-102
Average (%)
105
103
95
1:4
Range (%)
85-100
101-115
92-106
Average (%)
91
107
99
1:8
Range (%)
85-99
100-112
91-108
Average (%)
92
106
99
1:16
Range (%)
102-118
99-113
96-107
Average (%)
108
106
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-102
93
EDTA plasma (n=5)
100-111
105
Cell culture media (n=5)
89-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.59
295.15
892.74
91.31
286.62
892.52
Standard deviation
9.34
31.58
86.6
8.59
20.75
57.57
C V (%)
9.38
10.7
9.7
9.41
7.24
6.45
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PDPN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PDPN in samples. No significant cross-reactivity or interference between Human PDPN and analogues was observed.
