Human PECAM1/CD31 (Platelet/Endothelial Cell Adhesion Molecule 1) CLIA Kit
The Human PECAM1 (CD31) CLIA Kit is specifically designed for the precise measurement of Platelet Endothelial Cell Adhesion Molecule 1 (PECAM1) levels in human samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.PECAM1 is a vital protein involved in platelet-endothelial cell adhesion and plays a crucial role in maintaining vascular integrity. Dysregulation of PECAM1 has been linked to various cardiovascular diseases, inflammatory conditions, and immune disorders, highlighting its significance as a potential biomarker for diagnostic and therapeutic development.
With its advanced technology and reliable performance, the Human PECAM1 (CD31) CLIA Kit is a valuable tool for researchers studying the role of PECAM1 in health and disease, providing insights into its implications in various pathological conditions.
Product Name:
Human PECAM1/CD31 (Platelet/Endothelial Cell Adhesion Molecule 1) CLIA Kit
SKU:
HUES00927
Target:
Human PECAM1/CD31 (Platelet/Endothelial Cell Adhesion Molecule 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PECAM1/CD31. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PECAM1/CD31 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PECAM1/CD31, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PECAM1/CD31. You can calculate the concentration of Human PECAM1/CD31 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-108
90-106
87-101
Average (%)
98
98
95
1:4
Range (%)
98-110
90-102
103-114
Average (%)
104
96
108
1:8
Range (%)
96-109
93-104
88-101
Average (%)
103
98
95
1:16
Range (%)
95-110
89-100
91-107
Average (%)
103
95
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-113
106
EDTA plasma (n=5)
99-111
106
Cell culture media (n=5)
98-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.33
93.7
439.51
50.72
85.66
477.9
Standard deviation
5.58
10.49
27.12
5.46
9.77
31.49
C V (%)
11.55
11.2
6.17
10.76
11.41
6.59
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PECAM1/CD31 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PECAM1/CD31 in samples. No significant cross-reactivity or interference between Human PECAM1/CD31 and analogues was observed.
