Human PF4V1 (Platelet Factor 4 Variant 1) CLIA Kit
The Human PF4V1 (Platelet Factor 4 Variant 1) CLIA Kit is specifically designed for the precise quantification of PF4V1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.PF4V1 is a variant of Platelet Factor 4, a protein with vital roles in blood coagulation and inflammation. Abnormal levels of PF4V1 have been implicated in various disorders, including thrombosis, heparin-induced thrombocytopenia, and cardiovascular diseases.
Studying PF4V1 levels can provide valuable insights into the pathophysiology of these conditions and aid in the development of targeted therapies.The Human PF4V1 CLIA Kit from AssayGenie provides researchers with a reliable tool to investigate PF4V1 levels in human samples with precision and accuracy. With its high performance and ease of use, this kit is an invaluable asset for advancing research in thrombosis, platelet function, and related fields.
Product Name:
Human PF4V1 (Platelet Factor 4 Variant 1) CLIA Kit
SKU:
HUES01166
Target:
Human PF4V1 (Platelet Factor 4 Variant 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PF4V1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PF4V1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PF4V1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PF4V1. You can calculate the concentration of Human PF4V1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
92-109
89-102
Average (%)
96
100
94
1:4
Range (%)
89-103
100-114
90-103
Average (%)
96
106
96
1:8
Range (%)
97-109
93-108
90-105
Average (%)
103
100
96
1:16
Range (%)
100-113
95-108
90-105
Average (%)
106
102
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
95
EDTA plasma (n=5)
86-99
91
Cell culture media (n=5)
92-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.99
2.08
6.99
1.0
2.22
6.63
Standard deviation
0.12
0.22
0.65
0.08
0.22
0.63
C V (%)
12.12
10.58
9.3
8.0
9.91
9.5
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PF4V1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PF4V1 in samples. No significant cross-reactivity or interference between Human PF4V1 and analogues was observed.
