The Human PGDS (Prostaglandin D Synthase) CLIA Kit is specifically designed for the precise measurement of PGDS levels in human biological samples including serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring dependable and consistent results for various research purposes.PGDS is an important enzyme involved in the synthesis of prostaglandin D2, a key mediator in inflammation and allergic responses.
Dysregulation of PGDS has been linked to various inflammatory disorders, making it a valuable biomarker for studying these conditions and exploring potential treatment options.With its reliable performance and broad applicability, the Human PGDS CLIA Kit is an essential tool for researchers seeking to delve deeper into the role of PGDS in health and disease. Visit the provided URL for more information and to order your kit today.
Product Name:
Human PGDS (Prostaglandin D Synthase) CLIA Kit
SKU:
HUES01016
Target:
Human PGDS (Prostaglandin D Synthase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PGDS. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PGDS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PGDS, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PGDS. You can calculate the concentration of Human PGDS in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
103-119
99-114
Average (%)
98
110
106
1:4
Range (%)
104-116
96-113
97-109
Average (%)
110
103
102
1:8
Range (%)
93-107
92-105
89-105
Average (%)
98
99
96
1:16
Range (%)
103-117
101-116
86-97
Average (%)
109
109
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-116
106
EDTA plasma (n=5)
85-97
90
Cell culture media (n=5)
92-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.05
130.4
428.49
42.57
127.23
414.76
Standard deviation
5.15
11.55
29.99
5.19
11.76
47.12
C V (%)
10.95
8.86
7.0
12.19
9.24
11.36
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PGDS concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PGDS in samples. No significant cross-reactivity or interference between Human PGDS and analogues was observed.
