The Human PGR (Progesterone Receptor) CLIA Kit is a highly reliable and sensitive assay designed for the precise detection of progesterone receptor levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it an essential tool for research in various fields.The progesterone receptor is a key factor in regulating hormone signaling and gene expression, playing a crucial role in reproductive health, cancer progression, and other physiological processes.
Studying progesterone receptor levels can provide valuable insights into hormone-related disorders and potential therapeutic interventions.With its high specificity and sensitivity, the Human PGR CLIA Kit is an indispensable tool for researchers and clinicians looking to investigate progesterone receptor levels and their implications in various health conditions. Order your kit now from Assay Genie to advance your research and discover new insights in progesterone receptor biology.
Product Name:
Human PGR (Progesterone Receptor) CLIA Kit
SKU:
HUES01184
Target:
Human PGR (Progesterone Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PGR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PGR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PGR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PGR. You can calculate the concentration of Human PGR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
97-114
91-107
Average (%)
96
105
97
1:4
Range (%)
84-96
86-99
86-101
Average (%)
91
93
93
1:8
Range (%)
93-103
104-120
85-97
Average (%)
98
110
90
1:16
Range (%)
93-107
86-101
101-113
Average (%)
99
93
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-114
108
EDTA plasma (n=5)
90-105
96
Cell culture media (n=5)
88-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
216.02
574.03
1511.73
215.94
556.81
1426.17
Standard deviation
18.53
42.48
173.4
20.38
54.79
97.12
C V (%)
8.58
7.4
11.47
9.44
9.84
6.81
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PGR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PGR in samples. No significant cross-reactivity or interference between Human PGR and analogues was observed.
