Human PGRP-S (Peptidoglycan Recognition Protein-S) CLIA Kit
The Human PGRP-S (Peptidoglycan Recognition Protein S) CLIA Kit is specifically designed for the accurate detection of PGRP-S levels in human biological samples. This kit offers high sensitivity and specificity, providing reliable and reproducible results for a variety of research applications.PGRP-S is a key protein involved in recognizing peptidoglycan, a component of bacterial cell walls. It plays a crucial role in innate immune responses to bacterial infections, making it an important biomarker for studying host defense mechanisms and developing antimicrobial therapies.
With its advanced design and ease of use, the Human PGRP-S CLIA Kit is a valuable tool for researchers looking to investigate the role of PGRP-S in infectious diseases and immune responses. Order your kit today and take your research to the next level.
Product Name:
Human PGRP-S (Peptidoglycan Recognition Protein-S) CLIA Kit
SKU:
HUES01077
Target:
Human PGRP-S (Peptidoglycan Recognition Protein-S)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PGRP-S. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PGRP-S and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PGRP-S, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PGRP-S. You can calculate the concentration of Human PGRP-S in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-115
90-102
100-115
Average (%)
108
97
107
1:4
Range (%)
95-108
91-104
98-115
Average (%)
100
98
105
1:8
Range (%)
91-108
99-112
98-114
Average (%)
99
105
104
1:16
Range (%)
102-118
96-112
104-115
Average (%)
110
102
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-106
96
EDTA plasma (n=5)
96-112
102
Cell culture media (n=5)
86-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
184.68
573.7
1913.9
196.5
592.2
1824.75
Standard deviation
17.12
63.68
180.86
19.22
52.0
132.66
C V (%)
9.27
11.1
9.45
9.78
8.78
7.27
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PGRP-S concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PGRP-S in samples. No significant cross-reactivity or interference between Human PGRP-S and analogues was observed.
