The Human PHB (Prohibitin) CLIA Kit is a reliable and accurate tool for measuring prohibitin levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides consistent and reproducible results, making it an essential asset for various research applications.Prohibitin is a multifunctional protein that plays a critical role in various cellular processes, including cell cycle regulation, mitochondrial function, and tumorigenesis.
Its dysregulation has been linked to several diseases, making it a valuable biomarker for studying conditions such as cancer, metabolic disorders, and aging-related diseases.Overall, the Human PHB CLIA Kit offers a convenient and precise method for investigating the role of prohibitin in cellular pathways and disease development, paving the way for new insights and potential therapeutic approaches.
Product Name:
Human PHB (Prohibitin) CLIA Kit
SKU:
HUES01180
Target:
Human PHB (Prohibitin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PHB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PHB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PHB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PHB. You can calculate the concentration of Human PHB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
88-99
91-103
Average (%)
97
94
98
1:4
Range (%)
100-111
97-111
94-108
Average (%)
105
105
101
1:8
Range (%)
96-108
89-101
100-116
Average (%)
102
96
107
1:16
Range (%)
96-113
95-108
96-112
Average (%)
104
101
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-107
98
EDTA plasma (n=5)
88-102
93
Cell culture media (n=5)
89-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
188.52
444.17
1380.42
185.56
469.27
1266.23
Standard deviation
15.27
47.35
119.68
21.32
41.58
111.55
C V (%)
8.1
10.66
8.67
11.49
8.86
8.81
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PHB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PHB in samples. No significant cross-reactivity or interference between Human PHB and analogues was observed.
