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Human Phospho-STAT5A (Y694) and Total STAT5A PharmaGenie ELISA Kit (SBRS1991)

SKU:
SBRS1991
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
Sandwich
  • Human Phospho-STAT5A Y694 and Total STAT5A PharmaGenie ELISA Kit SBRS1991
  • Human Phospho-STAT5A Y694 and Total STAT5A PharmaGenie ELISA Kit SBRS1991
€820
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Description

Human Phospho-STAT5A (Y694) and Total STAT5A PharmaGenie ELISA Kit (SBRS1991)

Product SKU:SBRS1991
Size:96T
Application:This ELISA kit recognizes Human STAT5A phosphorylated at site Tyrosine-694 as well as total STAT5A.
Uniprot:P42229
Gene ID:6776
Gene Names:STAT5 / STAT5A
Pathway:JAK/STAT Signaling
Synonyms:Signal transducer and activator of transcription 5A
Target Species:Human
Compatible Sample Types:Cell Lysates, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Semi-Quantitative
Storage/Stability:Upon receipt, the kit should be stored at -20°C. Please use within 6 months from the date of shipment.
  1. Pre-Coated 96-well Strip Microplate
  2. Wash Buffer
  3. Anti-Phospho Antibody
  4. HRP-Conjugated Secondary Antibody
  5. Assay Diluent
  6. TMB One-Step Substrate
  7. Stop Solution
  8. Lysis Buffer
  9. Positive Control Sample

Other materials and equipment required:

The Assay Genie Human Phospho-STAT5A (Y694) and Total STAT5A PharmaGenie ELISA Kit (SBRS1991) will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 ul of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 ul of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 ul of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ul of Stop Solution to each well.
  11. Read at 450 nm immediately.
Product ImageA431 cells were treated or untreated with EGF. Cell lysates were analyzed using this phosphoELISA and Western Blot.
Product ImageA431 cells were treated with recombinant human EGF at 37°C for 20 min. Cells were solubilzed at 4 x 107 cells/ml in lysis buffer. Serial dilutions of lysates were analyzed in this ELISA.

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