Human Phosphoinositide-3-Kinase Catalytic Delta Polypeptide (PIK3Cd) ELISA Kit
The Human Phosphoinositide 3-kinase catalytic delta polypeptide (PIK3CD) ELISA Kit is specifically designed for the precise measurement of PIK3CD levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it a valuable tool for various research applications.PIK3CD is a key enzyme in the phosphoinositide 3-kinase (PI3K) pathway, playing a critical role in cell growth, proliferation, and survival. Dysregulation of PIK3CD has been linked to various diseases, including cancer, inflammatory disorders, and immune-related conditions.
Therefore, detecting and monitoring PIK3CD levels can provide valuable insights into the pathogenesis of these diseases and facilitate the development of targeted therapies.Overall, the Human PIK3CD ELISA Kit is an essential resource for researchers seeking to investigate the role of PIK3CD in disease pathophysiology and explore its potential as a therapeutic target.
Product Name:
Human Phosphoinositide-3-Kinase Catalytic Delta Polypeptide (PIK3Cd) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
