Human Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a) ELISA Kit
The Human Phosphoinositide 3-Kinase Class 2 Alpha Polypeptide (PIK3C2A) ELISA Kit is specifically designed for the accurate detection of PIK3C2A levels in human samples, including serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, ensuring precise and reproducible results for a variety of research applications.PIK3C2A is a key enzyme involved in intracellular signaling pathways, particularly in regulating cell growth, survival, and metabolism. Dysregulation of PIK3C2A has been linked to various diseases, including cancer, diabetes, and autoimmune disorders, highlighting its importance as a potential therapeutic target and biomarker for disease progression.
By utilizing the Human PIK3C2A ELISA Kit, researchers can accurately measure PIK3C2A levels in biological samples, enabling a deeper understanding of its role in disease pathology and the development of targeted treatments. This kit provides a valuable tool for studying PIK3C2A function and its implications in various disease states.
Product Name:
Human Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
