The Human Phospholipase C Zeta-1 (PLCZ1) ELISA Kit is specifically designed for the accurate quantification of PLCZ1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, allowing for precise and reliable results that are crucial for a wide range of research applications.PLCZ1 is an important enzyme that plays a key role in regulating intracellular calcium levels and is essential for sperm activation during fertilization.
Dysregulation of PLCZ1 has been associated with male infertility and reproductive disorders, highlighting its significance as a biomarker for studying these conditions and potentially developing targeted therapies.Overall, the Human PLCZ1 ELISA Kit from Assay Genie is a valuable tool for researchers looking to accurately measure PLCZ1 levels in human samples and further explore its role in various biological processes and diseases.
Product Name:
Human Phospholipase C Zeta 1 (PLCz1) ELISA Kit
Product Code:
HUDL02330
Size:
96 Assays
Target:
PLCz1
Synonyms:
NYD-SP27, PLCzeta, Testis-development protein NYD-SP27, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
82-102
92
EDTA plasma (n=5)
90-101
95
Heparin plasma (n=5)
88-100
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
