The Human Proinsulin CLIA Kit is specifically designed for the quantitative detection of proinsulin levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results, making it ideal for a variety of research applications.Proinsulin is a key molecule in the regulation of glucose metabolism, serving as a precursor to insulin. Monitoring proinsulin levels is important in studying conditions such as diabetes and metabolic disorders, as well as in assessing insulin production and secretion.
This CLIA kit offers a simple and efficient means of measuring proinsulin levels, allowing researchers to gain valuable insights into the mechanisms of insulin regulation and glucose homeostasis. It is a valuable tool for investigating the pathophysiology of diabetes and related conditions, as well as for developing new therapeutic strategies.
Product Name:
Human PI (Proinsulin) CLIA Kit
SKU:
HUES01170
Target:
Human PI (Proinsulin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PI. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PI, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PI. You can calculate the concentration of Human PI in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-109
90-105
102-117
Average (%)
99
98
109
1:4
Range (%)
99-115
86-98
102-115
Average (%)
107
92
108
1:8
Range (%)
86-97
86-100
85-99
Average (%)
91
93
92
1:16
Range (%)
91-103
89-104
83-98
Average (%)
97
95
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
104
EDTA plasma (n=5)
88-100
93
Cell culture media (n=5)
85-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
221.7
348.03
1342.46
210.99
341.35
1375.11
Standard deviation
17.89
33.48
107.4
24.12
24.44
101.76
C V (%)
8.07
9.62
8.0
11.43
7.16
7.4
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PI concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PI in samples. No significant cross-reactivity or interference between Human PI and analogues was observed.
