Human PIBF (Progesterone-induced-blocking factor) ELISA Kit
The Human PIBF (Progesterone-Induced Blocking Factor) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of PIBF levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an excellent choice for a variety of research applications.PIBF is a crucial factor involved in the regulation of the immune system and plays a significant role in pregnancy maintenance and fetal development.
Dysregulation of PIBF levels has been implicated in conditions such as recurrent pregnancy loss and autoimmune disorders, making it a valuable biomarker for studying these conditions and developing potential therapies.With its high sensitivity and specificity, the Human PIBF ELISA Kit is a valuable tool for researchers looking to study the role of PIBF in various physiological and pathological processes.
Product Name:
Human PIBF (Progesterone-induced-blocking factor) ELISA Kit
SKU:
HUES02751
Target:
Human PIBF (Progesterone-induced-blocking factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PIBF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PIBF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PIBF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PIBF. You can calculate the concentration of Human PIBF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-101
93-109
85-99
Average (%)
92
100
91
1:4
Range (%)
85-101
81-93
86-102
Average (%)
92
88
94
1:8
Range (%)
90-106
83-93
81-94
Average (%)
97
88
87
1:16
Range (%)
95-108
84-99
86-101
Average (%)
100
90
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
100
EDTA plasma (n=5)
93-106
98
Cell culture media (n=5)
95-106
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.09
2.84
8.62
1.1
2.89
8.03
Standard deviation
0.06
0.14
0.46
0.08
0.14
0.31
C V (%)
5.5
4.93
5.34
7.27
4.84
3.86
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PIBF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PIBF in samples. No significant cross-reactivity or interference between Human PIBF and analogues was observed.
