Human PICP (Procollagen I C-terminal Propeptide) CLIA Kit
The Human PICP (Procollagen I C-terminal propeptide) CLIA Kit is specifically designed for the precise measurement of PICP levels in human serum, plasma, and cell culture supernatants. This advanced kit offers unparalleled sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.PICP is an important marker for collagen synthesis, reflecting the formation of type I collagen in the body.
It plays a vital role in connective tissue health and integrity, making it a key biomarker for studying conditions such as fibrosis, osteoporosis, and skin disorders. By accurately measuring PICP levels, researchers can gain valuable insights into the mechanisms of these conditions and explore potential therapeutic interventions.
Product Name:
Human PICP (Procollagen I C-terminal Propeptide) CLIA Kit
SKU:
HUES00169
Target:
Human PICP (Procollagen I C-terminal Propeptide)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PICP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PICP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PICP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PICP. You can calculate the concentration of Human PICP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-98
88-103
100-119
Average (%)
90
96
109
1:4
Range (%)
86-99
85-98
95-107
Average (%)
91
91
100
1:8
Range (%)
97-113
94-105
101-115
Average (%)
104
99
106
1:16
Range (%)
103-115
88-103
100-115
Average (%)
109
94
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
94
EDTA plasma (n=5)
91-104
97
Cell culture media (n=5)
89-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.69
97.37
447.91
59.36
94.99
426.57
Standard deviation
4.78
9.39
41.79
5.02
7.56
28.84
C V (%)
8.74
9.64
9.33
8.46
7.96
6.76
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PICP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PICP in samples. No significant cross-reactivity or interference between Human PICP and analogues was observed.
