Human PIGR/SC (Polymeric Immunoglobulin Receptor/Membrane Secretory Component) CLIA Kit
The Human PIGR (Polymeric Immunoglobulin Receptor) CLIA Kit is a powerful tool for the precise measurement of PIGR levels in human biological samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and reproducible results, making it an invaluable resource for a variety of research studies.The PIGR protein plays a critical role in mucosal immunity by facilitating the transport of immunoglobulins across epithelial cells. Dysregulation of PIGR expression has been linked to various inflammatory and infectious diseases, highlighting its importance as a potential therapeutic target.
By using the Human PIGR CLIA Kit, researchers can gain valuable insights into the role of PIGR in health and disease, paving the way for the development of innovative treatment strategies. Whether studying mucosal immunity, inflammatory disorders, or infectious diseases, this kit provides a reliable and accurate solution for measuring PIGR levels in human samples.
Product Name:
Human PIGR/SC (Polymeric Immunoglobulin Receptor/Membrane Secretory Component) CLIA Kit
SKU:
HUES00704
Target:
Human PIGR/SC (Polymeric Immunoglobulin Receptor/Membrane Secretory Component)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PIGR/SC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PIGR/SC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PIGR/SC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PIGR/SC. You can calculate the concentration of Human PIGR/SC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-109
103-120
96-111
Average (%)
103
109
102
1:4
Range (%)
97-113
86-98
93-105
Average (%)
103
93
98
1:8
Range (%)
95-106
103-119
94-109
Average (%)
100
109
102
1:16
Range (%)
99-113
93-107
87-99
Average (%)
106
100
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
103-117
108
EDTA plasma (n=5)
97-109
102
Cell culture media (n=5)
96-110
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
55.51
100.26
435.14
58.01
91.36
463.3
Standard deviation
6.08
7.9
43.6
7.05
7.52
35.12
C V (%)
10.95
7.88
10.02
12.15
8.23
7.58
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PIGR/SC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PIGR/SC in samples. No significant cross-reactivity or interference between Human PIGR/SC and analogues was observed.
