Human PIINP (Procollagen II N-Terminal ProPeptide) ELISA Kit
The Human PIINP (Procollagen II N-terminal propeptide) ELISA Kit is specifically designed for the accurate measurement of PIINP levels in human serum, plasma, and cell culture supernatants. This kit offers excellent sensitivity and specificity, ensuring precise and consistent results for various research applications.PIINP is a key marker for collagen synthesis and bone formation, making it a valuable tool for studying skeletal development, bone metabolism, and various bone-related disorders.
By accurately measuring PIINP levels, researchers can gain valuable insights into bone health and potential therapeutic interventions.With its high-quality components and easy-to-use protocols, the Human PIINP ELISA Kit from AssayGenie is a reliable choice for researchers looking to explore the role of PIINP in bone physiology and pathology. Trust in the accuracy and precision of this kit to advance your research and uncover new discoveries in the field of bone health.
Product Name:
Human PIINP (Procollagen II N-Terminal ProPeptide) ELISA Kit
SKU:
HUES01935
Target:
Human PIINP (Procollagen II N-Terminal ProPeptide)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PⅡNP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PⅡNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PⅡNP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PⅡNP. You can calculate the concentration of Human PⅡNP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-113
96-110
91-109
Average (%)
103
103
99
1:4
Range (%)
92-103
81-93
83-96
Average (%)
97
86
88
1:8
Range (%)
88-100
80-93
85-96
Average (%)
94
86
89
1:16
Range (%)
86-99
86-100
87-100
Average (%)
93
91
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
97
EDTA plasma (n=5)
88-101
95
Cell culture media (n=5)
92-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.6
8.48
47.24
4.58
9.11
49.38
Standard deviation
0.24
0.44
2.54
0.24
0.42
2.04
C V (%)
5.22
5.19
5.38
5.24
4.61
4.13
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human Pâ…¡NP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Pâ…¡NP in samples. No significant cross-reactivity or interference between Human Pâ…¡NP and analogues was observed.
