The Human PKP1 (Plakophilin 1) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of PKP1 levels in human biological samples. This kit is ideal for researchers studying the role of PKP1 in various biological processes, including cell adhesion, signaling, and gene expression regulation.Plakophilin 1 is a key component of desmosomes, important cell-cell adhesion structures in epithelial tissues. Dysregulation of PKP1 has been associated with various diseases, including skin disorders and certain types of cancer.
By accurately quantifying PKP1 levels, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify novel therapeutic targets.With its reliable performance and user-friendly protocol, the Human PKP1 CLIA Kit is an essential tool for advancing research in cell biology, cancer biology, and dermatology. Ensure precise and reproducible results with this cutting-edge assay kit from Assay Genie.
Product Name:
Human PKP1 (Plakophilin 1) CLIA Kit
SKU:
HUES01160
Target:
Human PKP1 (Plakophilin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PKP1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PKP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PKP1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PKP1. You can calculate the concentration of Human PKP1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
87-99
102-115
Average (%)
104
93
108
1:4
Range (%)
99-112
95-106
100-118
Average (%)
105
101
108
1:8
Range (%)
95-112
101-112
96-109
Average (%)
103
107
101
1:16
Range (%)
90-106
97-111
93-104
Average (%)
97
103
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
99
EDTA plasma (n=5)
97-110
104
Cell culture media (n=5)
90-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
183.04
560.29
1509.07
190.29
595.18
1423.0
Standard deviation
22.99
62.3
108.2
22.7
58.51
111.56
C V (%)
12.56
11.12
7.17
11.93
9.83
7.84
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PKP1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PKP1 in samples. No significant cross-reactivity or interference between Human PKP1 and analogues was observed.
