The Human PKR1 (Prokineticin Receptor 1) CLIA Kit is a reliable tool for accurate detection of PKR1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.PKR1 is a key receptor involved in numerous physiological processes, including inflammation, pain modulation, and circadian rhythm regulation. Dysregulation of PKR1 has been linked to various disorders such as migraines, inflammatory conditions, and cancer, highlighting its importance as a potential therapeutic target and biomarker for disease progression.
With the Human PKR1 CLIA Kit, researchers can gain valuable insights into the role of PKR1 in disease pathology and evaluate its potential as a therapeutic target for developing novel treatment strategies. Trust in the accuracy and reliability of this kit for your research needs.
Product Name:
Human PKR1 (Prokineticin Receptor 1) CLIA Kit
SKU:
HUES01014
Target:
Human PKR1 (Prokineticin Receptor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PKR1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PKR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PKR1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PKR1. You can calculate the concentration of Human PKR1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-117
99-116
91-106
Average (%)
109
107
98
1:4
Range (%)
90-104
99-114
95-109
Average (%)
97
106
103
1:8
Range (%)
101-116
92-108
91-107
Average (%)
108
99
98
1:16
Range (%)
84-95
96-111
87-99
Average (%)
90
104
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
101
EDTA plasma (n=5)
92-104
98
Cell culture media (n=5)
95-111
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
392.32
1184.42
2856.71
367.65
1070.1
2967.36
Standard deviation
45.51
139.29
299.1
42.76
108.08
248.66
C V (%)
11.6
11.76
10.47
11.63
10.1
8.38
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PKR1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PKR1 in samples. No significant cross-reactivity or interference between Human PKR1 and analogues was observed.
