Human PLA2G10 (Phospholipase A2, Group X) ELISA Kit
The Human PLA2G10 (Phospholipase A2 Group X) ELISA Kit is specifically designed for the accurate quantification of PLA2G10 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.PLA2G10, also known as Phospholipase A2 Group X, is an important enzyme involved in lipid metabolism and inflammatory processes. It plays a key role in various cellular functions and has been implicated in diseases such as inflammation, atherosclerosis, and cancer.
By measuring PLA2G10 levels, researchers can better understand its role in these conditions and potentially develop targeted therapies.Overall, the Human PLA2G10 ELISA Kit provides researchers with a valuable tool for studying the role of this enzyme in health and disease, allowing for insights into potential treatment strategies and biomarker discovery.
Product Name:
Human PLA2G10 (Phospholipase A2, Group X) ELISA Kit
SKU:
HUES02118
Target:
Human PLA2G10 (Phospholipase A2, Group X)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PLA2G10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PLA2G10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PLA2G10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PLA2G10. You can calculate the concentration of Human PLA2G10 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
97-112
93-105
Average (%)
97
105
100
1:4
Range (%)
92-103
86-98
88-100
Average (%)
98
92
94
1:8
Range (%)
87-98
80-95
87-103
Average (%)
93
86
94
1:16
Range (%)
86-100
81-94
86-100
Average (%)
93
86
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-107
98
EDTA plasma (n=5)
95-106
100
Cell culture media (n=5)
91-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.49
80.03
181.57
23.78
87.62
192.03
Standard deviation
1.51
3.35
6.39
1.47
5.02
9.62
C V (%)
5.92
4.19
3.52
6.18
5.73
5.01
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PLA2G10 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PLA2G10 in samples. No significant cross-reactivity or interference between Human PLA2G10 and analogues was observed.
