Human Platelet Derived Growth Factor C (PDGFC) ELISA Kit
The Human Platelet-Derived Growth Factor C (PDGF-C) ELISA Kit is a highly reliable and accurate tool for the precise measurement of PDGF-C levels in human serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring consistent and reproducible results for various research applications.PDGF-C is an important growth factor that plays a crucial role in cell proliferation, migration, and tissue regeneration. It is involved in various physiological and pathological processes, including wound healing, tissue repair, and the development of certain diseases such as cancer and fibrosis.
Studying the levels of PDGF-C can provide valuable insights into these processes and aid in the development of potential therapeutic interventions.Overall, the Human Platelet-Derived Growth Factor C (PDGF-C) ELISA Kit is a valuable tool for researchers looking to accurately measure PDGF-C levels and investigate its role in various biological processes and diseases. It is a reliable and efficient assay that can contribute to further advancements in the field of cell biology and biomedical research.
Product Name:
Human Platelet Derived Growth Factor C (PDGFC) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
