Human PLAUR/uPAR (Plasminogen Activator, Urokinase Receptor) CLIA Kit
The Human PLaur (Plasminogen Activator, Urokinase Receptor) CLIA Kit is a powerful tool for measuring PLaur levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers outstanding sensitivity and specificity, ensuring accurate and consistent results for various research applications.PLaur, also known as the Urokinase Receptor, plays a crucial role in the regulation of the plasminogen activation system, which is involved in processes such as cell migration, tissue remodeling, and cancer metastasis.
Abnormal PLaur expression has been implicated in a variety of diseases, including cancer and inflammatory disorders, making it an important target for biomarker research and therapeutic development.With its reliable performance and comprehensive compatibility, the Human PLaur CLIA Kit is an essential tool for researchers studying the role of PLaur in health and disease. Trust Assay Genie for high-quality assays that drive your research forward.
Product Name:
Human PLAUR/uPAR (Plasminogen Activator, Urokinase Receptor) CLIA Kit
SKU:
HUES00910
Target:
Human PLAUR/uPAR (Plasminogen Activator, Urokinase Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PLAUR/uPAR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PLAUR/uPAR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PLAUR/uPAR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PLAUR/uPAR. You can calculate the concentration of Human PLAUR/uPAR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-118
96-111
102-119
Average (%)
109
102
110
1:4
Range (%)
91-102
94-105
90-100
Average (%)
97
100
95
1:8
Range (%)
85-99
96-112
86-99
Average (%)
90
102
91
1:16
Range (%)
92-107
99-113
88-101
Average (%)
99
106
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
93
EDTA plasma (n=5)
91-103
97
Cell culture media (n=5)
88-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.35
153.38
428.54
58.05
159.01
409.38
Standard deviation
6.1
17.5
45.51
5.57
12.24
30.58
C V (%)
11.22
11.41
10.62
9.6
7.7
7.47
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PLAUR/uPAR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PLAUR/uPAR in samples. No significant cross-reactivity or interference between Human PLAUR/uPAR and analogues was observed.
