Human PLTP (Phospholipid Transfer Protein) CLIA Kit
The Human PLTP (Phospholipid Transfer Protein) CLIA Kit is a reliable and accurate tool for the quantification of PLTP levels in human samples, including serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, providing precise and reproducible results for a variety of research applications.PLTP is a key protein involved in lipid metabolism, playing a crucial role in the transfer of phospholipids between lipoproteins.
Dysregulation of PLTP levels has been linked to various metabolic disorders, including atherosclerosis and dyslipidemia. By measuring PLTP levels, researchers can gain valuable insights into these conditions and potential therapeutic targets.Overall, the Human PLTP CLIA Kit offers researchers a valuable tool for studying lipid metabolism and related disorders, providing accurate and reliable measurements to advance their research efforts.
Product Name:
Human PLTP (Phospholipid Transfer Protein) CLIA Kit
SKU:
HUES00960
Target:
Human PLTP (Phospholipid Transfer Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PLTP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PLTP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PLTP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PLTP. You can calculate the concentration of Human PLTP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-104
93-106
87-99
Average (%)
99
101
93
1:4
Range (%)
92-104
97-111
99-112
Average (%)
97
103
105
1:8
Range (%)
95-105
87-101
90-102
Average (%)
100
93
96
1:16
Range (%)
97-112
87-100
90-104
Average (%)
105
92
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-102
97
EDTA plasma (n=5)
100-112
105
Cell culture media (n=5)
97-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
199.55
546.94
1717.95
201.58
521.38
1706.79
Standard deviation
20.71
63.72
191.04
22.4
59.28
122.55
C V (%)
10.38
11.65
11.12
11.11
11.37
7.18
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PLTP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PLTP in samples. No significant cross-reactivity or interference between Human PLTP and analogues was observed.
