Human PLVAP (Plasmalemma Vesicle Associated Protein) CLIA Kit
The Human PLVAP (Plasmalemma Vesicle-Associated Protein) CLIA Kit is a powerful tool for detecting PLVAP levels in human samples, such as serum and plasma. This kit offers high sensitivity and specificity, providing accurate and reliable results for a variety of research applications.PLVAP is a key protein involved in controlling endothelial cell permeability and regulating the passage of molecules across blood vessels. Dysregulation of PLVAP has been implicated in various diseases, including cancer and inflammatory disorders.
Therefore, measuring PLVAP levels can be helpful in understanding disease pathogenesis and developing targeted therapies.With its advanced technology and user-friendly protocol, the Human PLVAP CLIA Kit from AssayGenie is a valuable resource for researchers studying vascular biology, inflammation, and disease mechanisms. Get accurate and reproducible results with this innovative kit.
Product Name:
Human PLVAP (Plasmalemma Vesicle Associated Protein) CLIA Kit
SKU:
HUES00633
Target:
Human PLVAP (Plasmalemma Vesicle Associated Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PLVAP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PLVAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PLVAP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PLVAP. You can calculate the concentration of Human PLVAP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
101-115
84-98
Average (%)
103
107
91
1:4
Range (%)
85-99
99-115
95-110
Average (%)
92
107
103
1:8
Range (%)
89-107
97-109
87-100
Average (%)
97
104
92
1:16
Range (%)
94-107
97-110
85-100
Average (%)
101
105
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
93
EDTA plasma (n=5)
92-106
100
Cell culture media (n=5)
85-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.6
177.55
877.12
106.17
160.25
887.11
Standard deviation
8.6
20.13
75.26
13.69
13.93
67.33
C V (%)
8.72
11.34
8.58
12.89
8.69
7.59
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PLVAP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PLVAP in samples. No significant cross-reactivity or interference between Human PLVAP and analogues was observed.
