Human pNF-H (Phosphorylated Neurofilament H) ELISA Kit
The Human Phosphorylated Neurofilament-H (pNF-H) ELISA Kit is specifically designed for the precise measurement of phosphorylated neurofilament-H levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Phosphorylated neurofilament-H is a key protein involved in the structure and function of neurons, particularly in the central nervous system.
Elevated levels of pNF-H have been linked to neurodegenerative diseases such as Alzheimer's and Parkinson's, making it a valuable biomarker for studying these conditions and potentially developing new treatments.Overall, the Human Phosphorylated Neurofilament-H ELISA Kit provides researchers with a powerful tool for investigating the role of this important protein in neurological disorders and advancing our understanding of these complex diseases.
Product Name:
Human pNF-H (Phosphorylated Neurofilament H) ELISA Kit
SKU:
HUES03513
Target:
Human pNF-H (Phosphorylated Neurofilament H) ELISA Kit
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human pNF-H. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human pNF-H and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human pNF-H, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human pNF-H. You can calculate the concentration of Human pNF-H in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-96
89-103
95-112
Average (%)
90
97
103
1:4
Range (%)
89-101
81-94
83-94
Average (%)
94
87
88
1:8
Range (%)
88-102
83-96
86-99
Average (%)
96
89
91
1:16
Range (%)
94-105
79-92
87-97
Average (%)
100
85
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
91
EDTA plasma (n=5)
96-108
101
Cell culture media (n=5)
95-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
248.23
475.34
1765.4
238.06
485.7
1771.88
Standard deviation
16.46
26.71
62.5
15.28
20.35
78.32
C V (%)
6.63
5.62
3.54
6.42
4.19
4.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human pNF-H concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human pNF-H in samples. No significant cross-reactivity or interference between Human pNF-H and analogues was observed.
