The Human POMC (Pro-Opiomelanocortin) CLIA Kit is a cutting-edge assay designed for the precise measurement of POMC levels in human samples, including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an indispensable tool for a variety of research applications.POMC is a key precursor protein involved in the regulation of various physiological processes, including metabolism, immunity, and stress response. Dysregulation of POMC expression has been linked to a range of disorders, including obesity, adrenal insufficiency, and skin pigmentation disorders, highlighting its importance as a biomarker for disease research and therapeutic development.
The Human POMC CLIA Kit from AssayGenie provides researchers with a reliable and efficient solution for quantifying POMC levels, facilitating deeper insights into the role of this critical protein in health and disease. Unlock new possibilities for your research with this innovative assay.
Product Name:
Human POMC (Pro-Opiomelanocortin) CLIA Kit
SKU:
HUES00599
Target:
Human POMC (Pro-Opiomelanocortin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human POMC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human POMC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human POMC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human POMC. You can calculate the concentration of Human POMC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-111
85-99
89-102
Average (%)
101
91
94
1:4
Range (%)
100-112
94-110
86-97
Average (%)
106
101
91
1:8
Range (%)
99-110
88-99
98-115
Average (%)
105
93
105
1:16
Range (%)
93-108
101-115
89-102
Average (%)
99
108
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
97
EDTA plasma (n=5)
94-107
101
Cell culture media (n=5)
90-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
111.1
236.43
880.16
117.42
226.88
807.21
Standard deviation
10.65
20.83
68.21
13.94
19.49
81.85
C V (%)
9.59
8.81
7.75
11.87
8.59
10.14
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human POMC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human POMC in samples. No significant cross-reactivity or interference between Human POMC and analogues was observed.
