Human Postacrosomal Sheath WW Domain Binding Protein (PAWP) ELISA Kit
The Human Postacrosomal Sheath WW Domain Binding Protein (PAWP) ELISA Kit is a specialized assay designed for the precise quantification of PAWP levels in human samples, including serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring reliable and consistent results for various research applications.PAWP is a key protein involved in the fertilization process, playing a critical role in sperm-egg fusion and early embryo development.
Dysregulation of PAWP has been linked to infertility and reproductive disorders, making it a valuable biomarker for studying these conditions and exploring potential interventions.With its advanced technology and accurate performance, the PAWP ELISA Kit offers researchers a valuable tool for investigating the role of PAWP in human reproductive health and advancing our understanding of fertility-related disorders. Order yours today and take your research to the next level.
Product Name:
Human Postacrosomal Sheath WW Domain Binding Protein (PAWP) ELISA Kit
Product Code:
HUDL02195
Size:
96 Assays
Target:
PAWP
Synonyms:
WBP2NL, WBP2 N-Terminal Like, WW domain-binding protein 2-like
Detection Method:
Sandwich
Range:
31.25-2000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
