The Human POSTN/OSF-2 (Periostin) ELISA Kit is expertly crafted for the precise quantification of Periostin (POSTN/OSF-2) levels in various biological samples. Periostin is a multifunctional matrix protein crucial in tissue repair, cell adhesion, and tissue remodeling processes, making it a key player in extracellular matrix regulation and various pathophysiological conditions. Accurate measurement of POSTN/OSF-2 levels is essential for understanding its role in tissue homeostasis, inflammation, and disease progression, providing valuable insights for targeted intervention strategies.
With exceptional sensitivity and specificity, our POSTN/OSF-2 ELISA Kit ensures accurate and reproducible results, meeting the highest standards of quality control. Designed for robust performance and user-friendly operation, this kit is ideal for cutting-edge research applications. Trust Assay Genie's POSTN/OSF-2 ELISA Kit for dependable quantification of this critical biomarker in your studies.
Product Name:
Human POSTN/OSF-2 (Periostin) ELISA Kit
SKU:
AEES00164
Target:
Human POSTN/OSF-2 (Periostin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to POSTN/OSF-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for POSTN/OSF-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain POSTN/OSF-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of POSTN/OSF-2. You can calculate the concentration of POSTN/OSF-2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
88-102
94-109
Average (%)
94
93
100
1:4
Range (%)
88-103
86-98
93-107
Average (%)
95
93
100
1:8
Range (%)
92-106
87-102
94-107
Average (%)
97
94
100
1:16
Range (%)
84-96
86-100
90-103
Average (%)
91
92
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
94
EDTA plasma (n=5)
91-102
96
Cell culture media (n=5)
96-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
0.98
4.2
0.47
0.95
4.35
Standard deviation
0.03
0.04
0.13
0.03
0.05
0.15
C V (%)
6.3
4.0
3.09
5.38
5.43
3.34
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of POSTN/OSF-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes POSTN/OSF-2 in samples. No significant cross-reactivity or interference between POSTN/OSF-2 and analogues was observed.
