The Human PP (Pancreatic Polypeptide) CLIA Kit is a reliable and accurate tool for detecting pancreatic polypeptide levels in human samples. This kit is designed for use with serum, plasma, and cell culture supernatants, providing researchers with a versatile solution for their studies. Pancreatic polypeptide is a peptide hormone secreted by the pancreas, playing a role in regulating food intake and energy balance. Abnormal levels of pancreatic polypeptide have been associated with conditions such as obesity and diabetes, making it a valuable biomarker for research in these areas.
With high sensitivity and specificity, this CLIA kit delivers consistent and reproducible results, giving researchers confidence in their data. Whether studying metabolic disorders or investigating pancreatic function, the Human PP CLIA Kit is a valuable tool for advancing research in the field.
Product Name:
Human PP (Pancreatic Polypeptide) CLIA Kit
SKU:
HUES01172
Target:
Human PP (Pancreatic Polypeptide)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PP. You can calculate the concentration of Human PP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
94-108
85-97
Average (%)
105
101
90
1:4
Range (%)
100-113
100-112
103-119
Average (%)
106
107
110
1:8
Range (%)
86-99
101-116
100-113
Average (%)
93
107
107
1:16
Range (%)
85-98
92-106
86-99
Average (%)
91
99
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
91
EDTA plasma (n=5)
87-98
92
Cell culture media (n=5)
101-118
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.81
88.61
422.95
52.54
81.84
381.27
Standard deviation
4.52
8.2
29.35
5.64
9.32
33.06
C V (%)
8.72
9.25
6.94
10.73
11.39
8.67
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PP in samples. No significant cross-reactivity or interference between Human PP and analogues was observed.
