The Human PP13 (Placental Protein 13) CLIA Kit is a cutting-edge assay designed for the precise quantification of PP13 levels in human biological samples. This kit offers exceptional sensitivity and selectivity, guaranteeing precise and consistent results across a variety of research settings.PP13 is a key protein present in the placenta, playing a vital role in pregnancy and fetal development. It serves as a valuable biomarker for monitoring pregnancy-related complications such as preeclampsia and intrauterine growth restriction.
Understanding PP13 levels can provide important insights into pregnancy health and identify potential risks for maternal and fetal well-being.With its advanced technology and reliable performance, the Human PP13 CLIA Kit is an indispensable tool for researchers studying reproductive health, pregnancy complications, and placental biology. It offers unparalleled accuracy and precision in PP13 detection, enabling groundbreaking discoveries and advancements in the field of obstetrics and gynecology.
Product Name:
Human PP13 (Placental Protein13) CLIA Kit
SKU:
HUES01074
Target:
Human PP13 (Placental Protein13)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PP13. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PP13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PP13, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PP13. You can calculate the concentration of Human PP13 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-111
90-106
86-100
Average (%)
101
98
91
1:4
Range (%)
101-116
101-116
92-106
Average (%)
106
107
99
1:8
Range (%)
102-117
92-109
89-99
Average (%)
110
99
94
1:16
Range (%)
93-108
91-102
99-116
Average (%)
100
96
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
95
EDTA plasma (n=5)
91-103
96
Cell culture media (n=5)
96-111
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.79
44.86
171.48
25.99
49.14
163.05
Standard deviation
2.0
3.85
15.72
2.47
3.52
16.09
C V (%)
8.41
8.58
9.17
9.5
7.16
9.87
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PP13 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PP13 in samples. No significant cross-reactivity or interference between Human PP13 and analogues was observed.
