Human PRB1 (Basic Salivary Proline Rich Protein 1) CLIA Kit
The Human PRB1 (Salivary Proline-Rich Protein 1) CLIA Kit is specifically designed for the accurate measurement of PRB1 levels in human saliva samples. This kit offers high sensitivity and specificity, ensuring precise and dependable results for various research applications.PRB1 is a proline-rich protein found in saliva, playing a role in various oral functions such as lubrication, antibacterial activity, and taste perception.
Studies have shown potential implications of PRB1 in oral health and diseases, making it a valuable biomarker for investigating oral conditions and developing targeted treatments.Overall, the Human PRB1 CLIA Kit provides researchers with an efficient tool for quantifying PRB1 levels in saliva samples, contributing to a better understanding of oral health and disease mechanisms.
Product Name:
Human PRB1 (Basic Salivary Proline Rich Protein 1) CLIA Kit
SKU:
HUES00645
Target:
Human PRB1 (Basic Salivary Proline Rich Protein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PRB1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PRB1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PRB1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PRB1. You can calculate the concentration of Human PRB1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
91-101
96-114
Average (%)
92
96
104
1:4
Range (%)
90-100
98-112
87-99
Average (%)
95
105
93
1:8
Range (%)
91-106
95-109
104-117
Average (%)
99
101
110
1:16
Range (%)
93-108
91-103
88-102
Average (%)
100
97
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
92
EDTA plasma (n=5)
98-114
107
Cell culture media (n=5)
85-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.27
195.41
972.04
88.44
178.36
973.83
Standard deviation
10.4
14.03
90.79
8.55
16.8
97.97
C V (%)
11.39
7.18
9.34
9.67
9.42
10.06
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PRB1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PRB1 in samples. No significant cross-reactivity or interference between Human PRB1 and analogues was observed.
