Human PRB2 (Basic Salivary Proline Rich Protein 2) CLIA Kit
The Human PRB2 (Salivary Proline-Rich Protein 2) CLIA Kit is a cutting-edge assay designed for the precise measurement of PRB2 levels in human saliva samples. This advanced kit offers superior sensitivity and specificity for accurate and dependable results, making it perfect for various research applications.PRB2 is a key protein found in saliva that plays a crucial role in maintaining oral health and promoting overall well-being. It is involved in functions such as antimicrobial activity, wound healing, and taste perception, making it a valuable biomarker for studying oral diseases, immunity, and sensory perception.
By using the Human PRB2 CLIA Kit, researchers and healthcare professionals can gain valuable insights into the role of PRB2 in oral health and disease, paving the way for the development of innovative treatments and interventions. Trust in the precision and reliability of this kit to advance your research and improve patient outcomes.
Product Name:
Human PRB2 (Basic Salivary Proline Rich Protein 2) CLIA Kit
SKU:
HUES00654
Target:
Human PRB2 (Basic Salivary Proline Rich Protein 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PRB2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PRB2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PRB2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PRB2. You can calculate the concentration of Human PRB2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-111
100-116
86-100
Average (%)
101
108
93
1:4
Range (%)
85-101
99-117
87-100
Average (%)
92
107
92
1:8
Range (%)
96-112
99-113
103-120
Average (%)
102
104
110
1:16
Range (%)
84-95
101-117
99-112
Average (%)
90
109
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-116
107
EDTA plasma (n=5)
90-105
97
Cell culture media (n=5)
88-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.95
148.39
477.86
55.21
147.3
457.85
Standard deviation
6.12
15.51
30.3
5.15
15.83
44.92
C V (%)
11.14
10.45
6.34
9.33
10.75
9.81
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PRB2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PRB2 in samples. No significant cross-reactivity or interference between Human PRB2 and analogues was observed.
