Human PRELP (Proline Arginine Rich End Leucine Rich Repeat Protein) CLIA Kit
The Human PRELP (Proline-Arginine Rich End Leucine-Rich Repeat Protein) CLIA Kit is specifically designed for the accurate detection of PRELP levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures reliable and reproducible results, making it a valuable tool for various research applications.PRELP is a key protein that is known to play a role in extracellular matrix organization and cell adhesion.
Dysregulation of PRELP has been linked to various musculoskeletal disorders, such as osteoarthritis and osteoporosis. Therefore, the measurement of PRELP levels using this CLIA kit can provide valuable insights into disease mechanisms and potential treatment strategies.Overall, the Human PRELP CLIA Kit offers a reliable and efficient method for studying the role of PRELP in health and disease, making it a valuable asset for researchers and clinicians alike.
Product Name:
Human PRELP (Proline Arginine Rich End Leucine Rich Repeat Protein) CLIA Kit
SKU:
HUES00286
Target:
Human PRELP (Proline Arginine Rich End Leucine Rich Repeat Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PRELP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PRELP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PRELP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PRELP. You can calculate the concentration of Human PRELP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-100
92-103
95-109
Average (%)
95
98
103
1:4
Range (%)
98-113
93-105
97-109
Average (%)
106
99
102
1:8
Range (%)
102-114
85-101
92-105
Average (%)
107
92
98
1:16
Range (%)
93-106
83-99
98-110
Average (%)
100
90
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-106
100
EDTA plasma (n=5)
87-102
93
Cell culture media (n=5)
99-112
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
210.96
492.61
1838.33
204.5
491.9
1990.48
Standard deviation
22.26
53.94
181.08
16.48
56.17
222.14
C V (%)
10.55
10.95
9.85
8.06
11.42
11.16
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PRELP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PRELP in samples. No significant cross-reactivity or interference between Human PRELP and analogues was observed.
