The Human Prenylcysteine Oxidase 1 (PCYOX1) ELISA Kit is a reliable and sensitive assay designed for the accurate detection of PCYOX1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high specificity and precision, ensuring consistent and reproducible results for a variety of research applications.PCYOX1 is an important enzyme involved in the prenylation process, which plays a critical role in various cellular functions including protein trafficking and signal transduction.
Dysregulation of PCYOX1 has been implicated in diseases such as cancer, metabolic disorders, and neurodegenerative conditions, highlighting its significance as a potential biomarker for disease diagnosis and therapeutic development.By utilizing the Human PCYOX1 ELISA Kit, researchers can obtain valuable insights into the role of PCYOX1 in health and disease, ultimately advancing our understanding of cellular processes and paving the way for novel treatment strategies.
Product Name:
Human Prenylcysteine Oxidase 1 (PCYOX1) ELISA Kit
Product Code:
HUDL02217
Size:
96 Assays
Target:
PCYOX1
Synonyms:
PCL1, Prenylcysteine lyase
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
