The Human Preptin CLIA Kit is a specialized diagnostic tool designed for the precise measurement of preptin levels in human serum samples. This kit offers exceptional sensitivity and accuracy, providing reliable and consistent results for various research applications.Preptin is a peptide hormone that plays a crucial role in regulating glucose metabolism and bone growth. Abnormal levels of preptin have been linked to metabolic disorders such as diabetes and osteoporosis, making it an important biomarker for studying these conditions and developing targeted therapies.
With its user-friendly design and high-quality performance, the Human Preptin CLIA Kit is a valuable tool for researchers and clinicians seeking to better understand the role of preptin in human health and disease. Order now and unlock new possibilities in your research endeavors.
Product Name:
Human Preptin CLIA Kit
SKU:
HUES00544
Target:
Human Preptin
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Preptin. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Preptin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Preptin, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Preptin. You can calculate the concentration of Human Preptin in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-113
92-106
85-98
Average (%)
104
98
90
1:4
Range (%)
93-106
90-103
87-100
Average (%)
99
95
94
1:8
Range (%)
94-110
89-104
95-109
Average (%)
101
95
103
1:16
Range (%)
100-114
98-115
94-107
Average (%)
108
106
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
96
EDTA plasma (n=5)
92-104
98
Cell culture media (n=5)
87-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.15
141.21
433.63
51.42
154.12
471.37
Standard deviation
6.21
16.89
28.66
6.6
12.65
48.65
C V (%)
11.47
11.96
6.61
12.84
8.21
10.32
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Preptin concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Preptin in samples. No significant cross-reactivity or interference between Human Preptin and analogues was observed.
