The Human Preptin ELISA Kit is specifically designed for the precise measurement of preptin levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it perfect for various research applications.Preptin is a hormone that plays a vital role in regulating glucose metabolism and bone development. It is also known to impact insulin secretion and sensitivity, making it a valuable biomarker for studying metabolic disorders such as diabetes and osteoporosis.
By utilizing the Human Preptin ELISA Kit, researchers can gain valuable insights into the role of preptin in various physiological processes and its potential implications in disease development. This kit is an indispensable tool for advancing research in the fields of endocrinology and metabolic diseases.
Product Name:
Human Preptin ELISA Kit
SKU:
HUES02038
Target:
Human Preptin
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Preptin. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Preptin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Preptin, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Preptin. You can calculate the concentration of Human Preptin in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-110
89-104
94-109
Average (%)
101
95
101
1:4
Range (%)
93-105
83-94
86-99
Average (%)
98
87
92
1:8
Range (%)
90-103
85-100
86-97
Average (%)
95
92
91
1:16
Range (%)
94-106
84-97
88-101
Average (%)
100
88
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
94
EDTA plasma (n=5)
86-99
92
Cell culture media (n=5)
93-110
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
220.78
498.59
1351.02
229.51
525.86
1397.56
Standard deviation
12.41
23.08
62.82
16.04
27.56
42.35
C V (%)
5.62
4.63
4.65
6.99
5.24
3.03
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human Preptin concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Preptin in samples. No significant cross-reactivity or interference between Human Preptin and analogues was observed.
