The Human PRG4 (Proteoglycan 4) CLIA Kit is specially designed for the precise and reliable detection of PRG4 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.PRG4, also known as lubricin, is a key proteoglycan involved in joint lubrication and cartilage protection.
Its levels are important indicators of joint health and functionality, making it a valuable biomarker for studying conditions such as osteoarthritis and rheumatoid arthritis. Understanding PRG4 levels can provide insights into disease progression and potential therapeutic targets for managing joint-related disorders.
Product Name:
Human PRG4 (Proteoglycan 4) CLIA Kit
SKU:
HUES00940
Target:
Human PRG4 (Proteoglycan 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PRG4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PRG4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PRG4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PRG4. You can calculate the concentration of Human PRG4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
94-109
93-105
Average (%)
90
100
98
1:4
Range (%)
93-107
94-107
95-110
Average (%)
99
102
102
1:8
Range (%)
92-107
97-115
104-116
Average (%)
98
105
110
1:16
Range (%)
95-109
100-113
89-102
Average (%)
100
107
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-98
93
EDTA plasma (n=5)
94-107
102
Cell culture media (n=5)
100-115
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.94
3.21
9.81
0.98
2.92
10.44
Standard deviation
0.09
0.34
1.0
0.12
0.21
0.81
C V (%)
9.57
10.59
10.19
12.24
7.19
7.76
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PRG4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PRG4 in samples. No significant cross-reactivity or interference between Human PRG4 and analogues was observed.
